nmol l mitophagy dye Search Results


nmol l mitophagy dye  (Dojindo Labs)
95
Dojindo Labs nmol l mitophagy dye
Nmol L Mitophagy Dye, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmol+l+mitophagy+dye/MitoBright+LT+Green/pm41990575-259-2-11
Average 95 stars, based on 1 article reviews
nmol l mitophagy dye - by Bioz Stars, 2026-09
95/100 stars
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nmol l mtphagy dye  (Dojindo Labs)
96
Dojindo Labs nmol l mtphagy dye
Figure 4. Impact of miR-141 mimic and siMAPK1 on mitophagy flux: assessments under normal, starvation, and bafilomycin A treat ment conditions using <t>MtPhagy</t> and Lyso dyes (Dojindo) for mitophagy and lysosomes, respectively. (A) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in non-treated (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf), and miR-141 mimic transfected in low glucose (miR-141_starv) conditions, and after bafilomycin A treatment (miR-141_starv_Baf). (B) Median with error bars plot of the MFI data obtained from non-treated (nt), starvation (nt_starvation), star vation after bafilomycin A treatment (nt_starvation_Baf), miR-141 mimic transfected in low glucose (miR-141_starvation) conditions, and after bafilomycin A treatment (miR-141_starvation_Baf), and miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf). statistically significant differences (p < 0.05) are shown using asterisks. (C) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ baf), and siMAPK1 transfected in low glucose (siMAPK1_starv) conditions, and after bafilomycin A treatment (siMAPK1_starv_baf). (D) Median with error bars plot of the MFI data obtained from intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starvation_Baf), siMAPK1 transfected in low glucose (siMAPK1_starvation) conditions, and after bafilomycin A treat ment (siMAPK1_starvation_Baf), and siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ Baf). statistically significant differences (p < 0.05) are shown using asterisks. (E) and (F) Non-treated (nt), starved (starv), miR-141 mimic transfected in high glucose (miR-141) and miR-141 mimic transfected in low glucose (miR-141 starv) conditions PC3 cells, showing cell morphology, Lyso dye fluorescence (green) and Mtphagy dye fluorescence (red) on Etaluma LS620 inverted microscope. Cells with co-localisation are shown with white full triangle, cells with lacking colocalization are shown with transparent triangle. All microscopy images have a scale bar of 25 µm. Expression in (B) and (F) is assessed using the MFI parameter from FCS histograms, with values normalized to a control value of 1. Asterisks above bars denote statistically significant differences as determined by ANOVA (*p < 0.05).
Nmol L Mtphagy Dye, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmol+l+mitophagy+dye/Mitophagy+Detection+Kit/10__1080_slash_13102818__2023__2293055-98-7-10
Average 96 stars, based on 1 article reviews
nmol l mtphagy dye - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Figure 4. Impact of miR-141 mimic and siMAPK1 on mitophagy flux: assessments under normal, starvation, and bafilomycin A treat ment conditions using MtPhagy and Lyso dyes (Dojindo) for mitophagy and lysosomes, respectively. (A) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in non-treated (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf), and miR-141 mimic transfected in low glucose (miR-141_starv) conditions, and after bafilomycin A treatment (miR-141_starv_Baf). (B) Median with error bars plot of the MFI data obtained from non-treated (nt), starvation (nt_starvation), star vation after bafilomycin A treatment (nt_starvation_Baf), miR-141 mimic transfected in low glucose (miR-141_starvation) conditions, and after bafilomycin A treatment (miR-141_starvation_Baf), and miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf). statistically significant differences (p < 0.05) are shown using asterisks. (C) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ baf), and siMAPK1 transfected in low glucose (siMAPK1_starv) conditions, and after bafilomycin A treatment (siMAPK1_starv_baf). (D) Median with error bars plot of the MFI data obtained from intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starvation_Baf), siMAPK1 transfected in low glucose (siMAPK1_starvation) conditions, and after bafilomycin A treat ment (siMAPK1_starvation_Baf), and siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ Baf). statistically significant differences (p < 0.05) are shown using asterisks. (E) and (F) Non-treated (nt), starved (starv), miR-141 mimic transfected in high glucose (miR-141) and miR-141 mimic transfected in low glucose (miR-141 starv) conditions PC3 cells, showing cell morphology, Lyso dye fluorescence (green) and Mtphagy dye fluorescence (red) on Etaluma LS620 inverted microscope. Cells with co-localisation are shown with white full triangle, cells with lacking colocalization are shown with transparent triangle. All microscopy images have a scale bar of 25 µm. Expression in (B) and (F) is assessed using the MFI parameter from FCS histograms, with values normalized to a control value of 1. Asterisks above bars denote statistically significant differences as determined by ANOVA (*p < 0.05).

Journal: Biotechnology & Biotechnological Equipment

Article Title: Restoring mitophagy in prostate cancer cells: the role of miR-141 rescue in counteracting MAPK1/ERK2-dependent autophagy suppression

doi: 10.1080/13102818.2023.2293055

Figure Lengend Snippet: Figure 4. Impact of miR-141 mimic and siMAPK1 on mitophagy flux: assessments under normal, starvation, and bafilomycin A treat ment conditions using MtPhagy and Lyso dyes (Dojindo) for mitophagy and lysosomes, respectively. (A) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in non-treated (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf), and miR-141 mimic transfected in low glucose (miR-141_starv) conditions, and after bafilomycin A treatment (miR-141_starv_Baf). (B) Median with error bars plot of the MFI data obtained from non-treated (nt), starvation (nt_starvation), star vation after bafilomycin A treatment (nt_starvation_Baf), miR-141 mimic transfected in low glucose (miR-141_starvation) conditions, and after bafilomycin A treatment (miR-141_starvation_Baf), and miR-141 mimic transfected in high glucose (miR-141), and after bafilomycin A treatment (miR-141_Baf). statistically significant differences (p < 0.05) are shown using asterisks. (C) Flow cytometry histogram analysis of MtPhagy and Lyso dyes intensity in LNCaP and PC3 cells in intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starv_Baf), siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ baf), and siMAPK1 transfected in low glucose (siMAPK1_starv) conditions, and after bafilomycin A treatment (siMAPK1_starv_baf). (D) Median with error bars plot of the MFI data obtained from intact (nt), starvation (nt_starvation), starvation after bafilomycin A treatment (nt_starvation_Baf), siMAPK1 transfected in low glucose (siMAPK1_starvation) conditions, and after bafilomycin A treat ment (siMAPK1_starvation_Baf), and siMAPK1 transfected in high glucose (siMAPK1), and after bafilomycin A treatment (siMAPK1_ Baf). statistically significant differences (p < 0.05) are shown using asterisks. (E) and (F) Non-treated (nt), starved (starv), miR-141 mimic transfected in high glucose (miR-141) and miR-141 mimic transfected in low glucose (miR-141 starv) conditions PC3 cells, showing cell morphology, Lyso dye fluorescence (green) and Mtphagy dye fluorescence (red) on Etaluma LS620 inverted microscope. Cells with co-localisation are shown with white full triangle, cells with lacking colocalization are shown with transparent triangle. All microscopy images have a scale bar of 25 µm. Expression in (B) and (F) is assessed using the MFI parameter from FCS histograms, with values normalized to a control value of 1. Asterisks above bars denote statistically significant differences as determined by ANOVA (*p < 0.05).

Article Snippet: 500–563 nm), mitophagy was tracked with 100 nmol/L MtPhagy dye (Dojindo Molecular Technologies, MD01.5; Ex.

Techniques: Flow Cytometry, Transfection, Fluorescence, Inverted Microscopy, Microscopy, Expressing, Control